Coding

Part:BBa_K4759045:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


camA-RBS2-camB-linker-GFP1-10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1279
    Illegal BglII site found at 2292
    Illegal BamHI site found at 1273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1279
    Illegal AgeI site found at 37
    Illegal AgeI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Connecting GFP1-10 to the C terminus of camB by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.


Source

camA and camB from Pseudomonas putida

References